Apr 01, 1997 · e gene unc-76 (unc, uncoordinated) is necessary for normal axonal bundling and elongation wi in axon bundles in e nematode Caenorhabditis elegans. e UNC-76 protein and two human homologs identified as expressed sequence tags are not similar to previously characterized proteins and us represent a new protein family.Cited by: 219. e gene unc-76 is required for normal outgrow of axons of a number of neurons, pri ily in regions of neuron-neuron contact. We have reported at e gene encodes alternatively-spliced proteins, 385 and 376 amino acids long, at are not similar to any proteins in e Genbank database (WBG, 11(5), p. 31, 1991. 1991 C. elegans meeting abstracts, p. 57). UNC-76 homologs are essential for nervous system function in C. elegans, and e mammalian UNC-76 homolog FEZ1 is a direct target of protein kinase C (PKC)ζ-dependent signaling and axon outgrow (Bloom and Horvitz, 1997. Kuroda et al., 1999). Nor Carolina Triangle Area C. elegans labs. NC Triangle Worm Group. e next TWG meetings. TWG meetings are on a temporary hiatus. We shall return to e bowling alley meeting site some day. Local Labs UNC Chapel Hill Shawn Ahmed Rob Dowen Bob Goldstein. C. elegans strains. Strains of C. elegans were cultured at 20°C using standard protocols. e wild-type strain was N2 Bristol. Mutant alleles used in is study are listed below. LGI: ced-1(e1735), ced-12(n3261), unc- 8(n3263).LGII: laat-1(qx42), lgg-1(bp500).LGIII: ced-6(n2095), ced-7(n2094), ttr-52(tm2078), epg-3(bp405).LGIV: ced-2(n1994), ced-5(n1812), ced- (n3246). UNC-3 affects e development of e RID cell lineage. e C. elegans nervous system consists of 302 neurons of 118 subtypes, each wi distinct morphology, connectivity and position. One class, consisting of a single neuron, RID, is born during embryogenesis. e RID soma, which resides in e anterior dorsal ganglion, extends a single process at initially grows ventrally to join e. International C. elegans Conference GSA is proud to support e international community of C. elegans researchers and sponsors e International C. elegans Conference every two years.Attendees learn about cutting-edge research in a diverse array of topics, including: physiology, neurobiology, development, evolution, behavior, aging, ecology, gene regulation, genomics, and more. We analyzed e synaptic physiology of unc-13 mutants in e nematode C. elegans.Mutants of unc-13 had normal nervous system architecture, and e densities of synapses and postsynaptic receptors were normal at e neuromuscular ction. However, e number of synaptic vesicles at neuromuscular ctions was two- to reefold greater in unc-13 mutants an in wild-type animals. unc-76(e911) V. qxIs257. C. elegans: qxIs257 [ced-1p::nuc-1::mCherry + unc-76(+)]. Integrated nuc-1::mCherry transgene king lysosomes in epidermal cells. Site of chromosomal integration unknown. Not known if unc-76(e911) is still present in background. Reference: Li Y, et al. J Cell Biol. 24.215(2):167-185. XW8056: unc-76(e911) V. C. elegans mutants lacking e dense-core vesicle priming protein UNC-31 (CAPS) share highly similar phenotypes wi mutants lacking a neuronal Gαs pa way, including strong paralysis despite exhibiting near normal levels of steady-state acetylcholine release as indicated by drug sensitivity assays. Our genetic analysis shows at UNC-31 and neuronal Gαs are different parts of e same. «hide 20 30 40 50 msefsqtalf pslprtargt avvlgntpag dkiqycngts vytvpvgslt 60 70 80 90 0 dteiytehsh qttvaktsps gyycasgdvh gnvriwdttq t ilkttip 1 120 130 140 150 vfsgpvkdis wdseskriaa vgegrerfgh vflfdtgtsn gnltgqaram 160 170 180 190 200 nsvdfkpsrp friisgsddn tvaifegppf kfkstfgeht kfvhsvrynp 2 220 230 240 250 dgslfastgg dgtivlyngv dgtktgvfed dslknvahsg svfgltwspd 260 270 280 290 300. 12 International C. elegans Meeting e University of Wisconsin Word(s) to search for: Partial word matches allowed 25 unc-130 is required to maintain e correct expression pattern of unc-129 in C. elegans. 76 e Genetics of Ivermectin Resistance Joe . Structural component of e gap ctions (By similarity). Required for coordinated locomotion (PubMed:768 23). Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 in ALA neurons, involved in e rease in pharyngeal pumping during e quiescent state at precedes each larval molt (PubMed:17891142). 01, · C. elegans is a well-established genetic model and exhibits sequence similarity wi at least 42 of genes associated wi human disease (Culetto and Sattelle 2000). Additionally, e C. elegans nervous system is compact, wi only 302 neurons (White et al. 1986). C. elegans strains. General me ods for e maintenance of C. elegans have been described previously (Brenner, 1974). Worms were cultured on nematode grow medium (NGM) agar plates, plated wi Escherichia coli OP50 strain, at 20°C unless o erwise indicated. We . 25, 2006 · We have identified a el protein complex, composed of UNC-69 and UNC-76, which promotes axonal grow and normal presynaptic organization in C. elegans. As bo proteins are conserved rough evolution, we suggest at e mammalian homologs of UNC-69 and UNC-76 (SCOCO and FEZ, respectively) function similarly. e nematode C. elegans expressing e [Punc-25::GFP] transgene (juIs76). unc-25 encodes glutamic acid arboxylase (GAD). UNC-25 is expressed specifically in GABAergic neurons and localizes to cell bodies, axonal branches, and synaptic regions in e 19 type D motor-neurons, most notably shown here in e 13 ventral nerve cord neurons. 2000 East Coast C. elegans Meeting abstract 136: suppressor of daf-16 shortivity: age-n(b) 2000 East Coast C. elegans Meeting abstract 136: suppressor of daf-16 shortivity: che-2: X: 1.4: Apfeld & Kenyon 1999 abstract: che-3: I: 2: Apfeld & Kenyon 1999 abstract: che-11: V: 1.4: Apfeld & Kenyon 1999 abstract: che-13: I: 1.3: Apfeld & Kenyon 1999. 854 E.-C. PARK AND H. R. HORVJTZ unc- 5 is one of ese four genes wi an apparently wild-type null phenotype. In is paper, we present fur er phenotypic and genetic characterizations of unc- 5 mutants. MATERIALS AND ME ODS Strains and genetic nomenclature: Standard conditions for e culture, maintenance and e yl me anesulfonate (EMS) mutagenesis of C. elegans have been . 21, · Transformed E. coli OP50 expressing dsRNA targeting e Unc-22 gene were cultured wi C. elegans on NGM lite/amp+IPTG plates. 18, 1996 · C. elegans UNC-40, a transmembrane protein and DCC homolog, is required in motile cells for orienting tod UNC-6 netrin cues, and along wi UNC-5, for orienting away from ese same cues. While ese functional interactions could be mediated indirectly, e simplest model is at UNC-6 and UNC-5 interact wi UNC-40, i.e., as ligand. 01, ·, 1992 UNC-5, a transmembrane protein wi immunoglobulin and rombospondin type 1 domains, guides cell and pioneer axon migrations in C. elegans. Cell 71 . CEH-20/Pbx and UNC-62/Meis function upstream of rnt-1/Runx to regulate asymmetric divisions of e C. elegans stem-like seam cells. Saman a L. Hughes, Charles Brabin, Alison Woollard. 164- 11:24 Regulation of maternal Wnt mRNA translation in C. elegans embryos reveals mechanistic parallels between 3’ UTRs and transcription enhancers. Comamonas sp., a bacterium on which C. elegans grows particularly well. Str-R. DA1877 is derived from a bacterium isolated from soil in e Dallas area by Boris Shtonda in 2002. at strain was called H39 in Avery, L, Shtonda, BB (2003), Food transport in e C elegans pharynx, J Exp Biol 206: 2441-2457. unc-76 stands for e mutated hermaphrodite and e (+) stands for e wild type male. Unc-76 Unc-76 + Unc-76/ + Unc-76/ + + Unc-76/ + Unc-76/ + X Unc-76 X Unc-76 X+ X+/XUnc-76 X+/XUnc-76 O XUnc-76/O XUnc-76/O Once my group made e punett squares we concluded at if e mutation were autosomal dominant e worms would have 0 heterozygous genotype. e phenotype would be . West Coast Worm Meeting Abstracts. Meiotic Pairing and Synapsis [p 11] Amy MacQueen, Anne M. Villeneuve 2. Conditional mitotic spindle mutants in C. elegans [p 12] Pharyngeal extension: e short and e long of it [p 76] MF Portereiko, SE Mango 67. A VAB-8/UNC-51/UNC-14 complex mediates axon outgrow [p 77] Tina Lai, Gian Garriga 68. DNA clones from e unc-22 region (Moerman et al. 1986) to investigate e molecular organization of e gene and e nature of its product. In addition, because unc-22 is a frequent target of e C. elegans var. Bergerac mutator system, and because e mutator-induced al-. Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting xviii Presenter Underlined 72 Characterizing behavioral responses of adult nematodes to ascarosides Anna Hartmann, Michael O’Donnell, Dongshin Kim, Dirk Albrecht, Piali Sengupta 73 Genetic Analysis of C. elegans Pa ogen Avoidance Behavior Alexander Horspool, Hod Chang. unc-54 3 ′ UTR e 3 ′ UTR in pPD95.75 is derived from..pPD95.75 is derived from e muscle myosin heavy chain gene unc-54 and ensures efficient processing of transcripts. Some recen Transposons in C. elegans [HTML] Transposons in C. elegans: 1. Caenorhabditis elegans (/ ˌ s iː n oʊ r æ b ˈ d aɪ t ə s ˈ ɛ l ə ɡ æ n z /) is a free-living, transparent nematode, about 1 mm in leng, at lives in temperate soil environments. It is e type species of its genus. e name is a blend of e Greek caeno-(recent), rhabditis (rod-like) and Latin elegans (elegant). In 1900, Maupas initially named it Rhabditides elegans, Osche. e short coiled-coil domain-containing protein UNC-69 cooperates wi UNC-76 to regulate axonal outgrow and normal presynaptic organization in Caenorhabditis elegans. UNC-53 protein of C. elegans or its functional equivalent is identified as a signal transducer/integrator involved in controlling e rate and directionality of cell migration and/or cell shape. Nucleic acid sequences encoding UNC-53 protein or its functional equivalent, such as genomic or cDNA are used to transfect C. elegans or mammalian cell lines useful for identifying inhibitors. UNC-16/JIP3 and UNC-76/FEZ1 limit e density of mitochondria in C. elegans neurons by maintaining e balance of anterograde and retrograde mitochondrial transport Article Full-text available. A key goal of is session is to make C. elegans scientists ae of e issues and problems at parasitic nematode researchers 75 - 5:24 Mapping circuit-wide neuronal dynamics to a complex goal-directed behavior in C. elegans. Vladislav Susoy Harvard 76 - 5:36 Four glial cells regulate 168 - 9:06 UNC-119 is part of. 13, · We describe e molecular cloning and characterization of eunc-64 locus of Caenorhabditis elegans. unc-64 expresses ree transcripts, each encoding a molecule wi 63–64 identity to human syntaxin 1A, a membrane- anchored protein involved in synaptic vesicle fusion. Interestingly, e alternative forms of syntaxin differ only in eir C-terminal hydrophobic membrane . Animals have evolved diverse behaviors at serve e purpose of finding food in e environment. We investigated e food seeking strategy of e soil bacteria-eating nematode Caenorhabditis elegans. C. elegans bacterial food varies in quality: some species are easy to eat and support worm grow well, while o ers do not. We show at worms exhibit dietary choice: ey hunt for high quality. Conradt, B. & H.R. Horvitz (1999) e TRA-1A determination protein of C. elegans regulates ually dimorphic cell dea s by repressing e egl-1 cell dea activator gene. Cell. 98: 317–327. 8 urs Mosaicism Choate, K.A. et al. (20) Mitotic recombination in patients wi ich yosis causes reversion of dominant mutations in KRT. Stem cell niche exit in C. elegans via orientation and segregation of dhter cells by a cryptic cell outside e niche. eLife .9:e56383 DO I: .7554/eLife.56383. pdf. 19, · Xie, T. and Spradling C. (1998) apentaplegic is essential for e maintenance and division of germline stem cells in e Drosophila ovary. Cell 94: 251-260. 24 urs Epistasis and pa way analysis Conradt, B. & H.R. Horvitz (1999) e TRA-1A determination protein of C. elegans.